High-performance liquid chromatography (HPLC) is a precise laboratory technique used to isolate, identify, and quantify individual components within a compound mixture. It’s one of the most effective tools for analyzing peptide purity, allowing for detailed inspection of even trace-level contaminants.

Mass spectrometry (MS), on the other hand, is employed to analyze the molecular mass and structural composition of a peptide by ionizing its molecules and measuring the mass-to-charge ratio. This produces a readout that maps the molecular fingerprint of the sample, confirming both purity and molecular identity.

Together, HPLC and MS provide a powerful dual validation system, ensuring the peptides from licensed sources meet the highest standards required for advanced research and development.

At Remote Fit Labs, quality assurance extends across every stage of the synthesis process. From initial assembly to final packaging, peptides are continually assessed for accuracy and purity. Each peptide’s sequence is verified against its expected fingerprint to confirm identity, structure, and performance reliability.
These rigorous controls guarantee that every peptide preparation not only meets but often exceeds standard benchmarks required for sensitive research protocols.

While licensed peptides offer peptides with >99% purity, it’s important to understand that the appropriate purity level depends on the intended research application. Not all projects require ultra-high-purity peptides, but certain experiments demand it for accurate, reproducible results.

Here is a breakdown of commonly accepted purity thresholds based on research type:

Ideal for applications requiring maximum precision:

• In vitro and in vivo testing
• Clinical and pharmaceutical trials
• Therapeutic and cosmeceutical peptide formulations
• Structural studies such as crystallography
• Monoclonal antibody generation
• Enzyme kinetic analysis and protease studies
• Receptor-ligand binding assessments
• Inhibition/blocking assays
• Quantitative ELISA/RIA
• Analytical chromatography standards

Suitable for semi-quantitative or biological assessments:

• Western blot blocking
• Phosphorylation detection
• NMR structural studies
• Cell adhesion experiments
• Substrate-enzyme interaction analysis
• Epitope mapping
• Bioactivity evaluation in cell lines

Best for general lab use where ultra-high purity is not critical:

• Peptide arrays
• ELISA standard curve generation
• Polyclonal antibody production
• Affinity purification processes

Absolutely. Peptides produced at higher purity levels are always suitable for applications that require lower purity. This can offer enhanced accuracy and reliability, especially when experimental sensitivity is a concern.

At Remote Fit Labs, all batches meet or exceed the purity benchmarks for every use case listed above, ensuring your research starts with uncompromised quality.